Influence of adjuvants added to teflubenzuron spray on resistant and susceptible strains of the soybean looper Chrysodeixis includens (Lepidoptera: Noctuidae) / Influência da adição de adjuvantes à calda de pulverização de teflubenzuron em linhagens resistentes e suscetíveis da lagarta falsa-medideira Chrysodeixis includens em soja (Lepidoptera: Noctuidae)

The soybean looper (SBL), Chrysodeixis includens (Walker, [1858]) (Lepidoptera: Noctuidae), is a soybean and cotton pest in South America countries. Field-evolved resistance of SBL to inhibitors of chitin biosynthesis has been reported in Brazil; however, this mode of action is still widely used against SBL. On this basis, we conducted laboratory bioassays to investigate if adjuvants (Nimbus®, TA 35®, Break-Thru® S 240, and Rizospray Extremo®) added to the teflubenzuron spray increase the mortality of SBL strains (resistant, heterozygous, and susceptible to chitin biosynthesis inhibitors). Using chromatography analysis, we also evaluated the amount of teflubenzuron on soybean leaves when applied alone or in combination with adjuvants. In laboratory bioassays, the biological activity of teflubenzuron increased against the susceptible SBL strain when adjuvants were added. In contrast, no relevant effects of adjuvants added to the teflubenzuron spray against heterozygous and resistant SBL larvae were detected. In leaf bioassays, even leaves from the upper third part of the plants containing a significantly higher amount of teflubenzuron (3.4 mg/kg vs 1.7 and 0.6 mg/kg); the mortality of SBL strains was similar when teflubenzuron was applied alone or in mixture with adjuvants. Our findings indicated that adjuvants added to teflubenzuron spray do not provide a substantial increase in the mortality of SBL strains resistant to chitin biosynthesis inhibitors. Therefore, it is necessary to reduce the use of this mode-ofaction insecticide against SBL and to give preference to other insecticides or control tactic.

A lagarta falsa-medideira, Chrysodeixis includens (Walker, [1858]) (Lepidoptera: Noctuidae), é uma praga da soja e do algodão nos países da América do Sul. A resistência de C. includens a inibidores da biossíntese de quitina tem sido relatada no Brasil. Entretanto, esse modo de ação ainda é amplamente utilizado para controle de C. includens. Com base nisso, conduzimos bioensaios em laboratório para investigar se adjuvantes (Nimbus®, TA 35®, Break-Thru® S 240 e Rizospray Extremo®) adicionados à calda inseticida de teflubenzuron aumentam a mortalidade de linhagens de C. includens (resistentes, heterozigotos e suscetíveis a inibidores da biossíntese de quitina). Usando análise cromatográfica, também avaliamos a quantidade de teflubenzuron em folhas de soja quando aplicado isolado ou em combinação com adjuvantes. Em bioensaios de laboratório, a atividade biológica do teflubenzuron aumentou para a linhagem suscetível quando os adjuvantes foram adicionados à calda inseticida. Em contraste, nenhum efeito relevante de adjuvantes adicionados ao teflubenzuron foi detectado para os heterozigotos e resistentes. Em bioensaios de folhas, mesmo naquelas do terço superior das plantas, as quais apresentaram uma maior deposição de teflubenzuron (3,4 mg/kg vs 1,7 e 0,6 mg/kg); a mortalidade das linhagens de C. includens foi semelhante quando o teflubenzuron foi aplicado isolado ou com adjuvantes. Nossos resultados indicam que os adjuvantes adicionados ao teflubenzuron não fornecem um aumento substancial na mortalidade de linhagens de C. includens resistentes aos inibidores da biossíntese de quitina. Portanto, é necessário reduzir o uso desse modo de ação para o manejo de C. includens e dar preferência a outros inseticidas ou tática de controle.

A straightforward one-step strategy for SARS-CoV-2 diagnosis and screening of variants of concern: a multicentre study

BACKGROUND The prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) has changed unevenly over time around the world. Although whole genome sequencing is the gold standard for virus characterisation, the discovery of alpha VOC causing spike gene target failure (SGTF) result, when tested using an reverse transcription real-time polymerase chain reaction (RT-qPCR) assay, has provided a simple tool for tracking the frequencies of variants. OBJECTIVES The aim of this study was to investigate if a multiplex RT-qPCR assay (BioM 4Plex VOC) could be used to detect SARS-CoV-2 and to perform a VOC screening test in a single reaction tube. Here, we present the multicentre study evaluating this assay. METHODS Twelve laboratories have participated in the multicentre study. The BioM 4Plex VOC was distributed to them with detailed instructions of how to perform the test. They were asked to test the BioM 4Plex VOC in parallel with their routine Commercial SARS-CoV-2 diagnostic assay. Additionally, they were requested to select SARS-CoV-2-positive samples with genome sequenced and lineage definition according to PANGO lineage classification. FINDINGS The BioM 4Plex VOC and commercial RT-PCR assay are equally effective in detecting SARS-CoV-2. Results revealed a specificity of 96.5-100% [95% confidence interval (CI)], a sensitivity of 99.8-100% (95% CI), and an accuracy of 99.8-100% (95% CI). A 99% concordance rate was found between results from the BioM 4Plex VOC and that from available genome sequencing data. MAIN CONCLUSIONS The BioM 4Plex VOC provides an effective solution to detect SARS-CoV-2 infections and screening for VOCs in a single reaction. It is a straightforward method to help us monitor the frequency and distribution of VOCs and develop strategies to better cope with the pandemics.

A straightforward one-step strategy for SARS-CoV-2 diagnosis and screening of variants of concern: a multicentre study

BACKGROUND The prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) has changed unevenly over time around the world. Although whole genome sequencing is the gold standard for virus characterisation, the discovery of alpha VOC causing spike gene target failure (SGTF) result, when tested using an reverse transcription real-time polymerase chain reaction (RT-qPCR) assay, has provided a simple tool for tracking the frequencies of variants. OBJECTIVES The aim of this study was to investigate if a multiplex RT-qPCR assay (BioM 4Plex VOC) could be used to detect SARS-CoV-2 and to perform a VOC screening test in a single reaction tube. Here, we present the multicentre study evaluating this assay. METHODS Twelve laboratories have participated in the multicentre study. The BioM 4Plex VOC was distributed to them with detailed instructions of how to perform the test. They were asked to test the BioM 4Plex VOC in parallel with their routine Commercial SARS-CoV-2 diagnostic assay. Additionally, they were requested to select SARS-CoV-2-positive samples with genome sequenced and lineage definition according to PANGO lineage classification. FINDINGS The BioM 4Plex VOC and commercial RT-PCR assay are equally effective in detecting SARS-CoV-2. Results revealed a specificity of 96.5-100% [95% confidence interval (CI)], a sensitivity of 99.8-100% (95% CI), and an accuracy of 99.8-100% (95% CI). A 99% concordance rate was found between results from the BioM 4Plex VOC and that from available genome sequencing data. MAIN CONCLUSIONS The BioM 4Plex VOC provides an effective solution to detect SARS-CoV-2 infections and screening for VOCs in a single reaction. It is a straightforward method to help us monitor the frequency and distribution of VOCs and develop strategies to better cope with the pandemics.

"Yo fui violentada adentro, estando en un lugar que me tenían que cuidar": Experiencias de opresión y violencias en contextos de salud hacia mujeres con discapacidad y abordajes desde la terapia ocupacional feminista / "Eu fui violentada por dentro, estando em um lugar deveria ser cuidada": Experiências de opressão e violência em contextos de saúde em relação a mulheres com deficiências e abordagens da terapia ocupacional feminista / "I was raped inside, being in a place where I was supposed to be taken care of": Experiences of oppression and violence in health contexts towards women with disabilities and approaches from feminist occupational therapy

Resumen Introducción Las prácticas de violencia en contextos de salud constituyen una de las múltiples manifestaciones de la violencia contra las mujeres con discapacidad. En Chile, como en el resto del mundo, el desarrollo de estudios sobre estas violencias aun es incipiente. Objetivos Caracterizar prácticas de violencia hacia mujeres con discapacidad en contextos de salud, y caracterizar experiencias de reivindicación de derechos humanos de este colectivo de mujeres en Chile, desde las voces de activistas y profesionales terapeutas ocupacionales. Método Se realizó un análisis secundario de datos cualitativos de un estudio ejecutado entre los años 2015 y 2020. Desde un enfoque cualitativo y estudio colectivo de casos, se realizó un análisis temático secundario de los datos obtenidos de 8 entrevistadas. Resultados Desde la perspectiva de las informantes, las mujeres con discapacidad experimentan violencia estructural de forma sistemática y transversal, la que atraviesa otras diversas formas de violencia: física, psicológica, sexual, obstétrica y simbólica-institucional. Las experiencias de reivindicación de derechos humanos de este colectivo reflejan procesos de emancipación, resistencia y construcción de prácticas transformadoras de estas vulneraciones. Conclusión Las prácticas de violencia en contextos de salud hacia mujeres con discapacidad en Chile es una situación visualizada como manifestaciones de dominación y opresión contra ellas, que perpetúan su exclusión social y desigualdades en salud. Frente a esta situación de injusticia social, mujeres activistas y profesionales terapeutas ocupacionales plantean la necesidad de implementar estrategias de reivindicación de derechos humanos, junto a prácticas de resistencia colectiva.

Resumo Introdução As práticas de violência em contextos de saúde constituem uma das múltiplas manifestações de violência contra as mulheres com deficiências. No Chile, como no resto do mundo, o desenvolvimento de estudos sobre esta violência ainda é incipiente. Objetivos Caracterizar práticas de violência contra mulheres com deficiências em contextos de saúde e caracterizar experiências de reivindicações de direitos humanos deste grupo de mulheres no Chile, a partir das vozes de ativistas e terapeutas ocupacionais profissionais. Método Foi realizada uma análise secundária dos dados qualitativos de um estudo realizado entre 2015 e 2020. A partir de uma abordagem qualitativa e de um estudo de caso coletivo, foi realizada uma análise temática secundária dos dados obtidos de oito entrevistadas. Resultados Da perspectiva das informantes, as mulheres com deficiência experimentam a violência estrutural de forma sistemática e transversal, que atravessa outras formas de violência: física, psicológica, sexual, obstétrica e simbólica-institucional. As experiências deste grupo na reivindicação de seus direitos humanos refletem processos de emancipação, resistência e a construção de práticas que transformam estas violações. Conclusão As práticas de violência em contextos de saúde em relação às mulheres com deficiências no Chile é uma situação visualizada como manifestações de dominação e opressão contra elas, que perpetuam sua exclusão social e desigualdades na saúde. Diante desta situação de injustiça social, as mulheres ativistas e terapeutas ocupacionais profissionais propõem a necessidade de implementar estratégias para a reivindicação dos direitos humanos, juntamente com práticas de resistência coletiva.

Abstract Introduction The practices of violence in health contexts constitute one of the multiple manifestations of violence against women with disabilities. In Chile, as in the rest of the world, the development of studies on this violence is still incipient. Objectives To characterize practices of violence against women with disabilities in health contexts, and to characterize experiences of vindication of human rights of this group of women in Chile, from the voices of activists and professional occupational therapists. Method A secondary analysis of qualitative data from a study executed between 2015 and 2020 was conducted. From a qualitative approach and collective case study, a secondary thematic analysis of the data obtained from 8 interviewees was performed. Results From the perspective of the informants, women with disabilities experience structural violence in a systematic and transversal way, which crosses other various forms of violence: physical, psychological, sexual, obstetric, and symbolic-institutional. The experiences of this group in claiming their human rights reflect processes of emancipation, resistance, and construction of practices that transform these violations. Conclusion The practices of violence in health contexts toward women with disabilities in Chile is a situation visualized as manifestations of domination and oppression against them, which perpetuate their social exclusion and inequalities in health. Faced with this situation of social injustice, women activists and professional occupational therapists propose the need to implement strategies for the vindication of human rights, together with practices of collective resistance.

Caries dental y determinantes sociales de salud en niños de establecimientos educativos de los distritos de Canchaque y San Miguel de El Faique, provincia de Huancabamba, región de Piura, Perú, 2019 / Dental caries and social determinants of health in children of educational establishments of Canchaque and San Miguel de El Faique districts, Huancabamba province, Piura region, Perú, 2019

RESUMEN Introducción: el objetivo fue determinar la asociación entre Caries Dental (CD) y los Determinantes Sociales de la salud (DSS) en niños de establecimientos educativos de los distritos de Canchaque y San Miguel de El Faique, provincia de Huancabamba, región de Piura, Perú, año 2019. Métodos: estudio transversal. Muestra no probabilística, seleccionando 124 individuos de 11-12 años de sexto grado de primaria y primer grado de secundaria de las escuelas de los distritos de Canchaque y San Miguel de El Faique. Los DSS estuvieron compuestos por: género, ingreso económico, nivel de educación, condición de vivienda, empleo, saneamiento ambiental y acceso a los servicios de salud. La experiencia de CD fue medida mediante el índice CPOD. Se realizó un análisis bivariado empleando las pruebas estadísticas Chi cuadrado, U de Mann Whitney y Kruskal Wallis. Resultados: se encontró asociación entre prevalencia de caries dental y condición de vivienda (p<0,05), el índice de CPOD con género, nivel educativo, servicio higiénico y seguro de salud (p<0,05), y el índice ceod no presentó ninguna asociación con las variables (p>0,05). Conclusión: la caries dental tuvo asociación con los determinantes sociales de la salud de condición de vivienda, género, nivel educativo, servicio higiénico y seguro de salud.

ABSTRAC Introduction: the objective was to determine the association between Dental Caries (DC) and the Social Determinants of Health (SDOH) in children from educational establishments in the Canchaque and San Miguel de El Faique districts, Huancabamba province, Piura region, Peru, 2019. Methods: cross-sectional study. Non-probability sample, selecting 124 individuals from 11-12 years of sixth grade of primary and first grade of secondary from schools in the districts of Canchaque and San Miguel de El Faique, Huancabamba province, Piura-Peru, year 2019. The SDOH were made up of: gender, economic income, education level, housing condition, employment, environmental sanitation and access to health services. DC experience was measured using the DMFT Index. A bivariate analysis was performed using statistical tests Chi-Square, Mann Whitney U test y Kruskal Wallis Results: it was found association between to the prevalence of DC and housing condition (p<0.05), the DMFT index with gender, education level, hygienic service and health insurance (p<0.05), and dmft index there was no association with the variables (p >0.05). Conclusions: the dental caries was associated with SDOH of housing condition, gender, education level, environmental sanitation and health insurance.

Analytical and clinical performance of molecular assay used by the Brazilian public laboratory network to detect and discriminate Zika, Dengue and Chikungunya viruses in blood

ABSTRACT In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered at the Brazilian Regulatory Agency of Health Surveillance - ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their clinical similarities are challenges to correctly diagnose these viruses. The simultaneous detection of ZIKV, DENV and CHIKV is an important tool for diagnosis and surveillance. Here, we present the analytical and clinical performance evaluation of ZDC molecular assay (Bio-Manguinhos) at the public health laboratories three years after its registration at ANVISA. The clinical performance demonstrates the ZDC molecular assay (Bio-Manguinhos) has 100% sensitivity and 100% specificity to detect and discriminate ZIKV, CHIKV, and DENV from clinical plasma samples. The ZDC molecular assay (Bio-Manguinhos) results were highly reproducible and no cross-reactivity was seen during testing with a panel of other infectious agents. In conclusion, the ZDC molecular assay (Bio-Manguinhos) is an accurate and reliable tool to monitor Zika, dengue and chikungunya infections in countries like Brazil with simultaneous circulation of the three viruses.

Psicoeducación en salud mental: una herramienta para pacientes y familiares / Psychoeducation in mental health: a tool for families and patients

Las personas que padecen patologías de salud mental con frecuencia sufren estigma y exclusión por parte de la sociedad. El desconocimiento y el temor a ser discriminado evita el acercamiento de las personas a los servicios de salud, provocando latencias y abandono del tratamiento. La psicoeducación es una herramienta que busca que pacientes y familias conozcan la naturaleza de la enfermedad, promoviendo la autonomía, el empoderamiento y la reintegración social. El siguiente artículo recoge evidencia en torno a esta temática, discutiendo sobre la aplicabilidad de sus resultados, la importancia de replicar métodos y sistematizar experiencias.

Mental health illness are often related with social stigma. The unknowledge and the generalization of conducts of people with mental health illness, impacts the approach to social or medical services and sometimes quit the treatment. Psychoeducation as a tool in this area, persuits that patients and family know the nature of the illness, his ethiology, course, therapeutic approaches and outcomes. This article takes evidence sorround psychoeducation in mental health illness, discuss the appliance of the results and the importance of replying and systematization of experiences.

Atuação estratégica em direitos humanos contra o afastamento arbitrário de bebês do convívio familiar: reflexões sobre a prática / Strategic action on human rights against the arbitrary withdrawal of infantis from the family: reflections on the practice

O presente artigo oferece um relato da experiência de trabalho da Clínica de Direitos Humanos da UFMG, a partir do método da advocacia estratégica em direitos humanos, frente ao problema do afastamento arbitrário de recém-nascidos do convívio familiar e a sistemática de violações de direitos humanos e estigmatização enfrentada por mães em situação de vulnerabilização social no contexto da cidade de Belo Horizonte.(AU)

This article offers an experience report of the work developed by the UFMG Human Rights Clinic through the use of the strategic advocacy method in human rights, in order to approach the problem of arbitrary State removals of newborn children from their familiar atmosphere and the systematic human rights violations and stigmatization suffered by their vulnerable mothers, in the context of the city of Belo Horizonte.(AU)

One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil

ABSTRACT The Brazilian Public Health Service provides freely αPEG-IFN to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects the treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus (HCV) virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay and it has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit (Cobas/Taqman HCV Test v2.0 - Roche), showing virus load results with significant correlation between them (p= 0,012) using commercial and clinical panels. In addition, 611 samples from patients treated with peguilated alfa-interferon (αPEG-IFN) from different regions of Brazil were analyzed. Our one-step real-time RT-PCR assay demonstrated good performance in viral load measurement and in treatment course monitoring, with acceptable sensitivity and specificity values.

Voz e alteridade: um contraponto entre psicanálise e psicologias dialógicas / Voice and otherness: a counterpoint between psychoanalysis and dialogic psychologies / Voz y alteridad: un contrapunto entre el psicoanálisis y psicologías dialógicas

Este artigo apresenta e esquematiza um possível debate sobre a voz. Consideram-se contribuições psicanalíticas e construções produzidas por abordagens dialógicas em psicologia. Por meio da análise de textos considerados pioneiros em seus respectivos campos teóricos, destacam-se as confluências e divergências em relação ao endereçamento e à materialidade, eixos analíticos próprios a essas perspectivas. Em ambas a voz institui a subjetividade pela relação com o outro. Para a psicanálise essa questão amplia-se como consequência da articulação do apelo ao outro. Mediante a linguagem, a voz inscreve o sujeito no Outro e se atualiza como corpo. Irredutível à emissão vocal, a voz equivale a uma estrutura que pode apoiar-se em muitas experiências estéticas e sensoriais. Ao situar a voz no espaço topológico, a psicanálise lacaniana resolve aparentes paradoxos como o relativo à interioridade versus exterioridade.

This paper presents and outlines a possible debate about "voice". In order to do so, Psychoanalytical contributions and constructions produced by dialogical approaches in psychology are considered. The confluences and divergences related to "addressing" and "materiality", specific analytical axes to these perspectives, are contrasted through the analysis of texts considered pioneers in their respective theoretical fields. In both of them "voice" institutes the subjectivity by the relation with the other. For psychoanalysis this question is amplified as a consequence of the articulation of the appeal to the other. Through language, "voice" inscribes the subject in the Other and actualizes itself as a body. Irreducible to vocal emission, "voice" is equivalent to a structure that can draw on many aesthetic and sensory experiences. By situating "voice" in the topological space, Lacanian psychoanalysis resolves apparent paradoxes such as that related to interiority versus exteriority.

Este artículo presenta y esquematiza un posible debate sobre la voz. Son consideradas las contribuciones psicoanalíticas y las construcciones producidas por abordajes dialógicos en psicología. Por medio del análisis de textos considerados pioneros en sus respectivos campos teóricos, se destacan las confluencias y divergencias en relación al direccionamiento y a la materialidad, ejes analíticos propios a esas perspectivas. En ambas la voz instituye la subjetividad por la relación con el otro. Para el psicoanálisis esa cuestión se amplia como consecuencia de la articulación del apelo al otro. Por medio del lenguaje, la voz inscribe al sujeto en el Otro y se actualiza como cuerpo. Irreductible a la emisión vocal, la voz equivale a una estructura que puede apoyarse en muchas experiencias estéticas y sensoriales. Al situar la voz en el espacio topológico, el psicoanálisis lacaniano resuelve aparentes paradojas como lo relativo a la interioridad versus exterioridad.

MOLECULAR IDENTIFICATION OF Bartonella henselae IN A SERONEGATIVE CAT SCRATCH DISEASE PATIENT WITH AIDS IN RIO DE JANEIRO, BRAZIL / Identificação molecular de Bartonella henselae em paciente com SIDA soronegativo para doença da arranhadura do gato no Rio de Janeiro, Brasil

Bartonella henselae is associated with a wide spectrum of clinical manifestations, including cat scratch disease, endocarditis and meningoencephalitis, in immunocompetent and immunocompromised patients. We report the first molecularly confirmed case of B. henselae infection in an AIDS patient in state of Rio de Janeiro, Brazil. Although DNA sequence of B. henselae has been detected by polymerase chain reaction in a lymph node biopsy, acute and convalescent sera were nonreactive.

Bartonella henselae está associada a um amplo espectro de manifestações clínicas, incluindo a doença da arranhadura de gato, endocardite, e meningoencefalite, em pacientes imunocompetentes e imunocomprometidos. Relatamos o primeiro caso confirmado por método molecular de B. henselae em um paciente com SIDA no estado do Rio de Janeiro, Brasil. Apesar da sequência de DNA de B. henselae ser detectada pela reação em cadeia da polimerase em uma biópsia do linfonodo, soros das fases aguda e convalescente foram não reativos.

O método psicanalítico aplicado à pesquisa social: a estrutura moebiana da alteridade na possessão / The psychoanalytical method applied to social research: the moebian structure of alterity in possession / El método psicoanalítico aplicado a la investigación social: la estructura moebiana de la alteridad en la posesión

Tendo por finalidade explorar possibilidades de aplicação do método psicanalítico além da prática clínica, este trabalho propõe uma interpretação topológica lacaniana do fenômeno da possessão na umbanda. Visa-se apreender o sujeito para além do psiquismo individual, de forma que o espaço subjetivo compreenda - continuamente - a transição de identidades ocorrida no corpo do médium. Isso se sustenta pela concepção de que o sistema interpretativo (que organiza a relação entre o mundo dos espíritos e o mundo dos vivos) e o sujeito coexistem em uma mesma estrutura. Para tanto, parte-se do conceito de significante, por ele ser capaz de criar uma rede de sentidos em função do efeito de um dizer inconsciente. Mediante um estudo de caso, mostra-se como, na possessão, o sujeito - concomitantemente suposto agente e efeito da combinatória significante - apresenta-se tanto como médium quanto como espíritos possuidores, os quais constituem o avesso e o direito de uma estrutura unilateral.

Aiming to explore application possibilities of the psychoanalytical method farther than the clinical practice, this work proposes a Lacanian topological interpretation of the possession phenomenon in Umbanda. The subject is sought beyond the individual psychism in a way that the subjective space continually comprehends the identities transition occurred in the medium's body. This idea is sustained by the conception that the interpretative system (that organizes the relationship between the world of the spirits and the world of the livings) and the subject coexist in the same structure. Therefore, it starts from the concept of significant, as it is capable of creating a net of meanings according to the effect of an unconscious saying. Through a case study, it is shown how the subject, concomitantly supposed agent and effect of the significant combinatory, is presented in the possession as medium and as possessor spirits, which constitute the front side and the verso side of a unilateral structure.

Teniendo por finalidad emplear posibilidades de aplicación del método psicoanalítico además de la práctica clínica, este trabajo propone una interpretación topológica lacaniana del fenómeno de la posesión en la umbanda. Se busca aprehender el sujeto más allá del psiquismo individual, de modo que el espacio subjetivo comprenda - continuamente - la transición de identidades ocurrida en el cuerpo del médium. Eso se sostiene por la concepción de que el sistema interpretativo (que organiza la relación entre el mundo de los espíritus y el mundo de los vivos) y el sujeto coexisten en una misma estructura. Para tanto, se parte del concepto de significante, por este ser capaz de crear una red de sentidos en función del efecto de un decir inconsciente. Mediante un estudio de caso, se muestra, como en la posesión, el sujeto - concomitantemente supuesto agente y efecto de la combinatoria significante - se presenta tanto como médium como también como espíritus poseedores, los cuales constituyen el revés y el derecho de una estructura unilateral.

Segundo Webinar Observatorio de Escuelas Saludables: construyendo una vida saludable en la escuela y el hogar / Second Healthy Schools Observatory Webinar: Building a healthy life at school and at home

El siguiente trabajo se refiere a las relatorías de las ponencias presentadas en el Segundo Webinar denominado Seminario-Taller, "Construyendo una Vida Saludable en la Escuela y el Hogar", como parte de las actividades del Observatorio de Escuelas Saludables, en el marco del proyecto "Desarrollo de un modelo de ambientes escolares saludables sustentables" que ejecuta l a Universidad Autónoma del Estado de Hidalgo (México), la Secretaría Ejecutiva de Elige Vivir Sano del Ministerio de Desarrollo Social y Familia ( Chile), y la colaboración del I nstituto de Nutrición y Tecnología de l os Alimentos de la Universidad de Chile, con financiamiento del Fondo Conjunto de Cooperación Chile-México.